The Aprilia rider perfectly set himself up for qualifying by recording a lap of 2:01.563, securing the top spot. The conditions at the Circuit of The Americas changed significantly compared to Friday, with lower temperatures and increased wind, which influenced the riders' performance.

Ai Ogura suffered an early crash at Turn 20, but quickly recovered. Fabio Di Giannantonio set the early pace with a time of 2:01.983, but was overtaken by championship leader Marco Bezzecchi, who recorded 2:01.789.

Alex Marquez had a fast crash on entry to Turn 2, while Bezzecchi lost control at Turn 6. Jorge Martin joined the sub-2:02 group with a time of 2:01.996. Diogo Moreira also had an incident similar to Alex Marquez at Turn 2.

Marc Marquez went top with a time of 2:01.714, but Martin improved further, clocking 2:01.563. Francesco Bagnaia placed third with 2:01.763.

Ai Ogura suffered a second crash at Turn 2. At the end of the session, Bezzecchi placed fourth, followed by Di Giannantonio and Pedro Acosta in fifth and sixth place, respectively. Fermin Aldeguer continued his recovery from a leg injury, and Joan Mir finished in eighth place.

Alex Marquez and Raul Fernandez completed the top ten. Enea Bastianini finished in eleventh position, followed by Moreira and Brad Binder.

Luca Marini was in 14th place, ahead of Toprak Razgatlioglu and Ogura. Fabio Quartararo finished in 17th place, with a lap time of 2:03. Johan Zarco, Franco Morbidelli, Alex Rins, and Jack Miller rounded out the group of riders.

The session demonstrated the competitiveness and challenges of the circuit, with several incidents and changes in weather conditions affecting the riders' performance.

The Circuit of The Americas presented unique challenges due to the low temperatures and wind, causing several riders to struggle to maintain control of their bikes. The crashes of Alex Marquez and Bezzecchi, among others, underscored the demands of the circuit.

The practice session was crucial for riders to refine their strategies and prepare their bikes for qualifying, aiming to secure a good starting position for the race.